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Incharge (Pathology)
dasvnr@icmr.org.in, drvnrdas@yahoo.com
Fax: 0612-2634379

Dr. V. N.R. Das
A view of Pathology Laboratry
The Scientific Team
Name Qualification          Designation
Dr. V. N.R. Das              MBBS Scientist G
The Students Team
Name Qualification          Designation
Facilities & Services:

Routine Pathological investigations of ward and O.P.D. patients for diagnosis, treatment and follow up studies of kala azar/PKDL cases and other diseases. Investigations carried out are

  • Haematological: complete blood count ( TCDC, Hb%, Platelet count, peripheral smear comment, M.P., ESR etc.)
  • Splenic / Bone marrow aspiration smears staining and microscopy for detection of Leishmania donovani and cytology for exclusion of other diseases like malaria, leukaemia etc.
  • Routine urine examination.
  • Skin Biopsy collection from suspected PKDL cases:  Imprint smear preparation, staining & microscopy for Leishmania parasite demonstration for PKDL diagnosis, treatment and followup studies.
  • External Quality Control Assessment of Haematology & Transfusion Medicine ( ISHTM) and supported by NABL ( DST, Govt. of India). Regular internal quality control assessment of the haematological  tests  through BIORAD samples are also being done.
Existing infrastructure:
  • Automatic Blood Cell Counter for haematological tests ( Beckman Coulter Counter )
  • Binocular Transmitted Light Microscopes ( Zeiss, Leica, Prior).
  • ‘Variant’ Haemoglobin Testing System
  • Cold Centrifuge
  • Automatic Tissue Processor and Microtome.
  • Laminar Flow, etc.
Research Activities : 
  • Focussed on Clinicopathological aspects of visceral leishmaniasis ( VL or Kala azar) and Post kala-azar dermal Leishmaniasis (PKDL).
  • Types of anaemia with additional mechanism for its pathogenesis in Kala azar.

  • Bone Marrow Cytology  in VL to assess the response to specific therapy, changes in marrow cytology have been correlated  with the parasite load and haematological parameters in relation to treatment in both responsive and  unresponsive cases.

  • Clinicopathological changes in PKDL cases in relation to treatment and immune response with follow up studies.

  • Multidisciplinary field study on PKDL: covered 2500 population to find out its prevalence, incidence, other epidemiological & clinico-pathological features.

  • Immunohistochemical staining skin biopsy of PKDL lesions has been performed by me to find out its efficacy for detection the parasites antigens in tissues with low parasite density.

  • Immunophenotyping and Immuopathology of cellular infiltrates in dermal tissue and peripheral blood from PKDL cases to examine the distribution of different T-lymphocytes in their peripheral blood by using phenotype markers for CD4+ & CD8+ T cells and to correlate them with cytological changes

  • A Longitudinal field study for estimation of infection dynamics of L. donovani by Leishmanin and Direct Agglutination Test (DAT) tests  in the endemic community for kala azar conducted on KA endemic & non-endemic population, evaluated the current and post infectivity status in different specturm of kala-azar infection. The informations can be of immense use in estimating target population for active surveillance, chemotherapy or vaccination in disease control programme.
  • Regular internal and external quality control assessment of the laboratory tests are being done for the clinical drug trials being conducted at our institute.

  • In WHO/TDR/ICMR clinical  project on Miltefosine drug trial (Phase III-in children and phase IV) an open multicentric trial to assess the efficacy and safety for treatment of Visceral Leishmaniasis conducted in this institute conducted the laboratory investigations on VL cases at screening, during and after treatment with Miltefosine  and  follow up. Haematological tests, malaria parasite (M.P.) , Splenic/bone marrow aspiration’s microscopic examination for leishmania parasite detection and other pathological tests were performed in the trial to diagnose and assess the treatment response.

  • Above pathological investigations in phase III multicentric, randomised, controlled, clinical trial to assess the safety & efficacy of injectable drug Parmomycin in treatment VL cases ( by  Institute for One World Health  /  World Health Organisation ) conducted in our institute.

  • WHO/TDR/ ICMR  project entitled “ Safety and efficacy of oral Miltefosine in Patients with Post Kala azar dermal Leishmaniasis (PKDL )- dose findinding study comparing 8 and 12 weeks of treartment.  Performed Skin biopsy imprint / slit smear collection from PKDL patients with microscopy for parasite detection and other Pathological investigations before , during, after treatment and followup.
Ongoing Research Projects :
  1. Study of Imprint smear microscopy and PCR application on biopsy from dermal lesions for diagnosis of Post kala azar dermal leishmaniasis cases in Bihar.  
  2. Study on Immunopathology of Post Kala azar Dermal Leishmaniasis (PKDL): T-cell subsets.
  3. Study of Haemoglobinopathies in anaemia of kala-azar cases from Bihar.
Other Scientific & Academic Assignments:
  1. Training and guidance for short dissertation research work during summer training programmto the students of B.Sc. (Biotech), M.Sc. (Biotech), M.Sc. (Biochem.).

  2. Training for Good Laboratory Practices (GLP) and standard operative procedures  to technical staffs of department.
  1. Teaching to M.Sc. (Biochem.), M.Sc. (Biotech) students from different  universities during summer training programme at RMRI, Patna.

Guidance of research scholars

  1. Imparting training & guidance to the student working as S.R.F for Ph.D..in Pathology department at RMRI, Patna.

  2. Project Guidance to a   M.Pharm. student from NIPER, Hajipur.