Welcome to Library
Annual Reports
RMRIMS News Bulletine
Dr. Ashish Kumar
Scientist C
 
Date of Birth:
18-10-1981
Sex: Male
Date of Joining in ICMR: 23.01. 2019
Designation: Scientist-“C”
Discipline: Biochemistry
Date of Joining in Present Post:
23.01.19
Publications
   
Office
ICMR- Rajendra Memorial Research Institute of Medical Sciences, Department of Health Research,
Ministry of Health & Family Welfare,
Government of India, Agamkuan, Patna-800007
Telephone: 0612- 2631565
Fax: 0612-2634379
Residence: C/o Shri Satish Chandra Pathak,
Pharmaceutical Colony, Jai Prakash Nagar
East of Bhoothnath Road, Patna-800026
Mobile: 09852087462, 08210353361

E-Mail: ashish2k8@gmail.com, ashish.rmrims@icmr.gov.in
 
Educational Qualifications:
  • Ph.D. (2014): Biochemistry, Calcutta University,Kolkata.
  • M.Sc. (2006): Biochemistry (1st Div), Science College, Patna University, Patna, Bihar.
  • B. Sc. Hons. (2003): 1st Div.
 
Research Experience:
Post Organization Period
Post-Doctoral Fellow
ICMR-Rajendra Memorial Research Institute of Medical Sciences, Patna. 01.07.2014 - 31.06.2016
01.08.2016 – 31.12.2018
Scientist -II
ICMR-Rajendra Memorial Research Institute of Medical Sciences, Patna. 01.08.2016 – 31.12.2018
Scientist C ICMR-Rajendra Memorial Research Institute of Medical Sciences, Patna. 23-01-2019– till date
 
Membership/Fellowship:  
  • Indian Science congress (Membership no: L35679)
   
Awards:  
  • Awarded Senior Research Fellowship by theICMR-RMRIMS on Mechanism of Amphotericin B Resistance in Leishmania donovani parasitesfrom May2010 to April 2012.
  • Awarded Post-Doctoral Fellow by theICMR-RMRIMS on Ascorbate based regulation of intracellular redox metabolism in clinical isolates of AmB resistant L.donovani from July 2014 to June 2016.
  • Awarded Dr. D.S. Kothari Post-Doctoral Fellow by theUniversity Grants Commission(UGC) on January 2017.
   
Seminars/Symposiums/Workshops/Conference:  

1.

Ashish Kumar, S. Das and P.Das “ Ascorbate based regulation of Intracellular redox metabolism in clinical isolates of Amphotericin B Resistant Leishmania donovani” atImmunocon 2015, International conference organized by Rajendra Memorial Research Institute and Medical Sciences, Patna.

2.

Ashsih Kumar, Bidyut Purkait,S.Das,   Abul Hasan Sardar and P. Das “Studies on differentially expressed genes in Amphotericin B resistant and sensitive strain of leishmania donovani.”At 100 years of antimonials: An international conference on November23-25, 2012 at IICB, Kolkata.

3.

Ashsih Kumar, Bidyut Purkait,  Shusmita Das, Abul Hasan sardar, Manish Kumar,  D.Singh and P. Das “Ascorbate peroxidase overexpression protects drug resistance Leishmania donovani  cells against reactive oxygen species mediated by auto-oxidation of Amphotericin B” at Allahabad agriculture institute,Allahabad,6-8th 2012.

4.

Ashish Kumar, Paritosh Mishra, Bidyut Purkait,  S.Das,  and P. Das “Role of MDR1 in Amphotericin B resistant and sensitive strain of leishmania donovani” (2013) in National seminar organized by National Institute of Pharmaceutical Education & Research, Hajipur on 20th September 2013

5.

Ashish Kumar, Nilay Nandi, Abul Hasan sardar, Shusmita Das, Bidyut Purkaitand P. Das“Studies on differentially expressed genes in Amphotericin B resistant and sensitive strain of leishmania donovani using DNA Microarray, at RMRIMS on 10-11 November,2011.

6.

Bidyut Purkait, Nilay Nandi, Ashish Kumar, Abul Hasan Sardar, Sudeep Kumar,  Pradeep Das “Analysis of the Involvement of Thiol metabolic Pathway in Amphotericin B Resistance Mechanism for Leishmania donovani” at Research Training on Intracellular Pathogens", 2010 on 2-7 February,2010 at The International Centre, Goa.

7.

Abhik Sen, Rajdeep Banerjee, Nilay Nandi, Sudeep Kumar, Sushmita Das, Ashish Kumar, Dharmendra Singh, Pradeep Das “Differential Gene Expression in Leishmnia donovani amastigotes isolated from three different physiological conditions: some functional studies on Amastin protein” at World Leish 4 on February 3-7, 2009 at Lucknow, India.

   
Workshop:
  • Participated in two days’ workshop on Good Clinical Laboratory Practices (GCLP) from 22nd to 23rd June 2016 organized by DNDi.
  • Participated in two days workshop on Intellectual Property Rights (IPR), from 15th to 16th March 2016 sponsored by IPR unit, ICMR, Department of health & Research, Govt. of India, New Delhi.
  • Delivered lectures in two days workshop on “Application of Bioinformatics in Medical Sciences” on 8th& 9th April 2015.
  • Participated in Workshop on Medical Devices Regulations/DPCO 2013 held at Patna on 11th September 2013 organized by State drug controller, Bihar.
   
Major achievements:
  • Leishmaniasis treatment remains a challenge that needs further research to achieve more specific and efficient medicines. In the absence of a suitable vaccine, chemotherapy remains the only treatment option. We have reported Amphotericin B (AmB, the first line drug) resistant Kala-azar cases in the field and decoded for the first time the AmB resistance mechanism in clinical isolates of Leishmania donovaniusing Genomic DNA Microarray and proteomic serological strategies.We showed that altered membrane composition, ATP-binding cassette transporters and theup regulated thiol metabolic pathway have a role in conferring amphotericin B resistance in clinical isolates of Leishmania donovani.
  • We have also demonstrated thatoverexpression of Ascorbate Peroxidase (APx) in the L.donovani AmB resistant strain prevents cells from the deleterious effect of oxidative stress, i.e., mitochondrial dysfunction and cellular death induced by AmB. The absence of an LdAPx homologue in humans makes it an excellent drug target. Moreover, overexpression of LdAPx in the L.donovani AmB resistant strain prevents cells from the deleterious effect of oxidative stress. The absence of LdAPx homologue in humans makes it an excellent drug target. We have designed an inhibitor against the LdApx which may also act as the template for further drug development.