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Dr. Vahab Ali, Ph. D.
 
A Microscopic view of amastigotes of L.donovani in Bone-marrow aspirates
The division of Microbiology is mainly focused in different aspects of parasitic diseases which covers various area like host-parasites interaction, identifications of new strains, mechanism of drug resistance, Fe-S cluster biogenesis, parasites survival under-oxidative stress, new drug targets, mechanism of pathogenesis, development of new diagnostic tools, vaccine candidates of Leishmania parasites.
 
The Scientific Team
Name Qualification          Designation
Dr. Vahab Ali
M.Sc., M. Phil., Ph.D. (Biochemistry) Scientist 'E'
M.Sc., Ph.D. (Zoology) Scientist 'D'
Dr. Shubhankar Kumar Singh    
M.Sc., Ph.D. (Botany) Scientist 'D'
The Students Team

Name

Qualification         

Designation

Mr. Ajit Kumar Thakur

M.Sc. (Biotechnology)

SRF

Mr. Pushkar Shivam

M.Sc. (Biotechnology)

SRF

Ms. Fauzia Jamal

M.Sc. (Biotechnology)

SRF

Mr Kuljit Singh

M. Pharma. (Biotechnology)

SRF

Ms. Sarita Kumari

M.Sc. (Biotechnology)

SRF

Mr. Gufran Ahmad

M.Sc. (Biotechnology)

SRF

Mr. Manish Kumar

M.Sc. (Biotechnology)

SRF

Mr. Hara Prasad Mandal

M. Pharma. (Biotechnology)

SRF

Mr. Gaurav Garg

M. Pharma. (Biotechnology)

SRF

Ms. Pushpanjali

M.Sc. (Bimedical Genetics)

JRF

Mr. Amit Kumar Dubey

M. Pharma. (Biotechnology)

JRF

Miss. Shalini Yadav

M.Sc. (Biotechnology)

JRF

Mrs. Parool Gupta

M. Pharma. (Biotechnology)

DST Inspire fellow

 
Facilities & Services:
In vitro maintenance of different isolates of Leishmania parasites for various research activities.
Bacterial culture of urine and their drug sensitivity.
.
Culture of bone marrow or splenic aspirate and biopsy of skin lesion of KA & PKDL patients, respectively for the primary isolation of promastigotes.
Department of Microbiology has state of art infra-structure facilities (sophisticated instruments) to perform various biochemical, immunological, protein structure and function, Cell biology, molecular biology experiments for their research work
   
Existing infrastructure:
HPLC, PCR/RT-PCR
Spectrofluorimeter with plate reader
UV-visible Spectrophotometer
Fluorescence Inverted and upright microscopes
CO2 and BOD incubators, high speed centrifuges
SDS-PAGE, Agarose gel, ELISA Reader, Western blotting system
Various freezer (-80 °C & -30 °C), Auto clave, Bio-safety cabinets,

Chemi Gel-doc system, electroporator, water-bath incubators, lyophilizer

Other basic equipments required for biochemical, microbiological and immunological research
   
Major achievements
Showed diurnal periodicity of Leishmania amastigotes in peripheral blood of Kala-azar patients.
Developed sensitive media for primary isolation of L. donovani from bone marrow aspirate and developed completely autoclavable media for in vitro maintenance.
Developed a simple technique to remove contamination from Leishmania culture without using antibiotics / anti fungal agents.
First time demonstrated and characterized iron sulfur clusters (ISC) assembly in Leishmania parasites.
Established role of ISC machinery components (IscS, IscU, Isd11, & frataxin proteins) in parasites survival under-oxidative stress, iron deficient conditions and association of these proteins in AmpB resistance.

First time demonstrated cysteine synthase role in parasites survival under-oxidative stress and its association with AmpB resistance.

Role of thiol metabolic pathways proteins in drug resistance and identified new drug targets against leishmaniasis.
Preservation of L. donovani promastigotes by a simple method up to 7 months in blood agar slants.
A pilot study on the antileishmanial activity of metabolites of bacteria isolated from sand fly gut.

 
Ongoing Activities : 
Functional characterization of Fe-S cluster biogenesis pathways and role of Fe-S proteins in parasites survival, pathogenesis.

Mechanism of drug resistance, parasites survival under oxidative stress
Identification of new antileishmanial compounds, vaccine candidates, and development of new tools for VL & PKDL diagnosis
Functional analysis the role of thiol metabolic proteins in drug resistance to explore/identify novel drug targets against leishmaniasis
In vitro, Drug (SAG) sensitivity test on intracellular amastigotes of L. donovani and its comparison with clinical response of the similar isolates.
Crucial role of plants-extract for the culture of Leishmania donovani promastigotes.

Antileishmanial metabolites from the bacteria of sand fly (Phlebotomus argentipes) gut.
Use of clot or sedimented cells of human discard blood as an easily available & economical alternative to Fetal Bovine Serum or fresh rabbit blood in completely autoclavable medium for culture of Leishmania donovani promastigotes.
Identification of new antigens from immune complex to develop new diagnostic tools against leishmaniasis
Development of serum free medium for parasites/animal cells culture.